The strip documents the life, loves, and politics of a group of lesbians living in a medium-sized city in the US, featuring both humorous soap-opera storylines and biting topical commentary. The characters include:

• Mo, the central character, a politically-committed lesbian-feminist with a tendency to whine;
• Lois, a sex-positive activist and housemate to Ginger and Sparrow;
• Ginger, a struggling academic and professor;
• Sparrow, a women's shelter worker and New Ager, currently dealing with a relationship with a man, Stuart;
• Clarice, a workaholic environmental lawyer, her wife Toni, an accountant and homemaker, and their son Raffi;
• Sydney, an amoral yuppie academic, Mo's girlfriend; and
• Jezanna, manager of the late gay and lesbian bookstore Madwimmin Books, which also employed Mo and Lois.

• Dykes to Watch Out For (1986)
• More Dykes to Watch Out For (1988)
• New, Improved! Dykes to Watch Out For (1990)
• Dykes to Watch Out For: The Sequel (1992)
• Spawn of Dykes to Watch Out For (1993)
• Unnatural Dykes to Watch Out For (1995)
• Hot, Throbbing Dykes to Watch Out For (1997)
• The Indelible Alison Bechdel: Confessions, Comix, and Miscellaneous Dykes to Watch Out For (1998)
• Split-Level Dykes to Watch Out For (1998)
• Post-Dykes to Watch Out For (2000)
• "Dykes and Other Carbon-Based Life-Forms to Watch Out For" (2003)

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